Effect of Notch2 in chondrocytes

To investigate the role of Notch2 in the inflammatory response in chondrocytes, we cultured primary chondrocytes derived from wild type and Notch2 mutant mice and treated TNFalpha or vehicle. We then performed gene expression profiling analysis using data obtained from RNA-seq of wild type and Notch2 mutant chondrocytes. Overall design: Comparative gene expression profiling analysis of RNA-seq data for wild type and Notch2 mutant chondrocytes with/without TNFalpha