Analysis of relative stabilities of overall model proteins for sampling sites.

Panel (a) is a plot of the measured temperatures as a function of distance with a smooth curve connecting the points. Panel (b) is a plot of the relative stabilities of the overall model proteins as a function of and temperature at the pH of site 3 (7.933). Panel (c) is a plot of the measured pHs as a function of distance with a smooth curve connecting the points. Panel (d) is a plot of the relative stabilities of the overall model proteins as a function of and pH at the temperature of site 3 (67.5°C). The dashed lines in (b) and (d) depict Eq. (24). Panel (e) is a plot of the relative stabilities of the overall model proteins as a function of at the temperature and pH of site 3. Panel (f) is a plot of the relative stabilities of the overall proteins as a function of the combination of changes with distance of measured temperature and pH and modeled (using Eq. 24).