Calcitriol and hyperthermia potentiate gemcitabine efficacy – A Multifactorial Preclinical Evaluation in Pancreatic and Breast Cancer - replication data

This project supports a comprehensive evaluation of the therapeutic potential and synergy between gemcitabine, calcitriol, and hyperthermia in the treatment of pancreatic and breast cancer. The dataset encompasses a wide range of quantitative and qualitative measurements obtained from both in vitro and in vivo models, including human and murine pancreatic cancer cells (PANC-1, Panc02) and murine breast cancer cells (4T1), as well as a syngeneic mouse model bearing orthotopic pancreatic tumors. Data types include cell viability and proliferation (MTT assays, manual cell counts), apoptosis and necrosis markers (flow cytometry), protein expression profiles (Western blot), live-cell imaging (fluorescence microscopy), tumor growth monitoring (ultrasound imaging), and hematological parameters (blood morphology). Treatments were applied as single agents or in dual and triple combinations, and experiments were conducted across multiple timepoints to assess both immediate and long-term effects. This multidimensional dataset enables a detailed analysis of treatment efficacy, potential synergistic or additive effects, and systemic toxicity, offering valuable insight into the biological mechanisms underlying multimodal cancer therapy and its translational relevance.