Immunoassay and proteomics dataset: Identification and validation of urine CXCL-9 as a biomarker for diagnosis of acute interstitial nephritis

Background: Acute tubulointerstitial nephritis (AIN) is one of the few causes of acute kidney injury with diagnosis-specific treatment options. However, due to the need to obtain a kidney biopsy for histological confirmation, AIN diagnosis can be delayed, missed, or incorrectly assumed. Here we identify and validate urine CXCL-9, an interferon-γ-induced chemokine involved in lymphocyte chemotaxis, as a diagnostic biomarker for AIN.
Methods: In a prospectively-enrolled cohort with pathologist-adjudicated histological diagnoses (discovery cohort), we tested the association of 180 immune proteins measured by an aptamer-based assay with AIN and validated the top protein, CXCL-9, using sandwich immunoassay. We externally validated these findings in 2 cohorts with biopsy-confirmed diagnoses (validation cohorts) and examined mRNA expression differences in kidney tissue from patients with AIN and controls.
Results: In aptamer-based assay, urine CXCL-9 was 7.6-fold higher in AIN than controls (P..., , , Immunoassay and proteomics dataset: Identification and validation of urine CXCL-9 as a biomarker for diagnosis of acute interstitial nephritis

10.5061/dryad.ksn02v788

\## Description of the Data and file structure

Cross-sectional data from Yale AIN study including participants who underwent a kidney biopsy at two healthcare centers

Observations: 249

Variables: 26 13 Oct 2020 13:42

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Variable Storage Display Value

name type format label Variable label

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sequenceno str17 %17s sequenceno

diagnosis_histo byte %22.0g diagnosis1\_

Diagnosis1

ain_prebx byte %8.0g ain_prebx\_

Was AIN suspected before biopsy?

ain_...