Transcriptional profiling of p53 mutants in KP LUAD cells. Transcriptional profiling of p53 mutants in KP LUAD cells

Transcriptional profiling of KP LUAD cells expressing a panel of p53 mutant cDNAs. Overall design: 1 million KP LUAD cells were seeded in 10cm plates and immediately transduced with retroviral supernatants and 8ug/mL polybrene. Cells were grown for 48hrs before purifying using fluorescence activated cell sorting (FACS). All transductions were done in triplicate. Following transduction, stable GFP+ populations were purified by FACS on a FACSAria (BD Biosciences). 120 hours post-transduction, total RNA was isolated using the RNeasy Mini Kit (Qiagen) following standard manufacturer protocols.