MicroRNA expression profile of different grade pancreatic neuroendocrine tumors

The grade of pancreatic neuroendocrine tumors principally influences therapy. Grading is currently done by the histological examination of biopsy samples, which is an invasive and time-consuming method. We performed an NGS on FFPE tumor tissue samples to find microRNAs that show a significant difference in expression according to grade, thus could be suitable for grade determination. Overall design: Tumor tissue was signed and macrodisected from FFPE blocks by pathologists to make sure the samples we used contain solely tumor tissue. We isolated total RNA from the samples