Life stage specific transcriptomes and methylomes from A. pisum bacteriocytes
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA613906
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资源简介：
To better understand the establishment and regulation of the symbiotic relationship between the pea aphid A. pisum and its obligate endosymbiont Buchnera, in general and throughout aphid nymphal development, bacteriocytes were dissected from clonal females at five different life stages: 1st instar, 2nd instar, 3rd instar, 4th instar nymph, young pre-reproductive adult. Bacteriocytes were dissected and pooled from over 100 individual aphids per life stage and from 3 biological sub-lines per life stage. DNA and RNA were co-collected, cleaned, purified, and concentrated. After passing QA/QC standards, RNA samples were polyA purified, converted into libraries, and sequenced (150PE) on a HiSeq4000. DNA samples were spiked with lambda phage DNA, bi-sulfate converted into libraries, and sequenced (PE150) on NovaSeqS4.
创建时间：
2020-03-22
