Longitudinal tropism testing results per subject.^{^a^,^b^,^c}

aESTA, Enhanced-Sensitivity Trofile™ Assay; Pop Seq, population sequencing of the V3-loop; 454, 454 sequencing of the V3-loop; MT2, direct co-cultivation of patient-derived peripheral blood mononuclear cells with MT-2 cells. HIV-1 RNA levels are in copies/mL; CD4+ cell counts are in cells/mm3. bPopulation and 454 sequencing data shown here used the Geno2Pheno[coreceptor] false positive rate cut-off providing highest accuracy when assigning HIV-1 tropism, i.e.: 20% and 10%, respectively. Based on internal error controls, only V3 forms present in ≥0.6% of viruses were considered for tropism prediction with 454 sequencing. cTests detecting CXCR4-using HIV are reported as “dual-mixed, DM” for ESTA, “X4” for population sequencing, “percent of X4 viruses” for 454, and “syncytium-inducing, SI” for MT-2 assays; for clarity, viruses only using CCR5 are shown as dashes; NA, tropism test result not available due to lack of amplification.