The coverage of the protein-coding sequence of each gene of interest is tabulated, as a percentage, for a range of sequencing depths (≥1x, 10x, 20x, 30x, 50x and 100x reads).
“Frequency” shows the numbers of flies homozygous and heterozygous for each gene. A χ2-test was conducted under the expectation of a 1:1 segregation ratio between homozygous and heterozygous flies.
