Genome-wide identification of altered m6A-related transcript profiles for vascular tissue in septic rats

Sepsis is the leading cause of death in hospital intensive care units. Recent studies have shown that N6-methyladenosine (m6A) modification promotes the inflammatory response, and we evaluated the effect of lipopolysaccharide (LPS) on aortic m6A modifications in mRNAs and lncRNAs. LC-MS/MS was used to verify the m6A global level of LPS and Control groups. High-throughput microarray was performed on the aortic tissue to identify m6A differentially methylated lncRNAs and mRNAs. m6A single-base site qPCR analysis was used to verify the microarray data. Gene Ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to investigate the biological functions. Establishment of competing endogenous RNA (ceRNA) and protein-protein interaction were performed to study the molecular interactions of these molecules. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis was used to verify the m6A modification related genes.