Pervasive phylogenomic incongruence underlies evolutionary relationships in eyebrights (Euphrasia, Orobanchaceae)

Disentangling the phylogenetic relationships of taxonomically complex plant groups is often mired by challenges associated with recent speciation, hybridisation, complex mating systems and polyploidy. Here, we perform the first global phylogenomic analysis of eyebrights (Euphrasia), a group renowned for taxonomic complexity, with the aim of understanding the evolutionary processes underlying phylogenetic discordance. We generate whole genome sequencing data and integrate this with prior genomic data to perform a comprehensive analysis of nuclear genomic, nuclear ribosomal (nrDNA), and complete plastid genomes from 57 individuals representing 36 species sampled across the genus. The species tree analysis of 3454 conserved nuclear scaffolds (46 Mb) is structured by geography and ploidy, and partially by taxonomy, and indicates that post glacial colonisation of North Western Europe occurred in multiple waves from discrete source populations. However, most species are not monophyletic, and ..., Our phylogenomic analyses included a total of 58 samples, 56 samples from 36 Euphrasia species and two outgroup species, Bartsia alpina and Neobartsia chilensis. This included a combination of newly sequenced samples and previously generated data. Short-read Illumina data for each sample was used for de novo plastid genome assembly using Novoplasty or GetOrganelle, nuclear ribosomal DNA array assembly using Novoplasty, and nuclear genome analysis based on mapping to the Euphrasia arctica genome., , # Pervasive phylogenomic incongruence underlies evolutionary relationships in eyebrights (Euphrasia, Orobanchaceae)

[https://doi.org/10.5061/dryad.jh9w0vtd3](https://doi.org/10.5061/dryad.jh9w0vtd3)

## Description of the data and file structure

Our phylogenomic analyses included a total of 58 samples, 56 samples from 36 *Euphrasia* species and two outgroup species, *Bartsia alpina* and *Neobartsia* *chilensis*. This included a combination of newly sequenced samples and previously generated data. Short-read Illumina data for each sample was used for de novo plastid genome assembly using Novoplasty or GetOrganelle, nuclear ribosomal DNA array assembly using Novoplasty, and nuclear genome analysis based on mapping to the Euphrasia arctica genome. 

### Files and variables

#### File: ConservedScaffolds.tree

**Description:** Sequence analysis of conserved nuclear genome scaffolds, used to make Figure 3 in the paper.

#### File: mash\_NJ.treefile

**Description:** Neighbor-joining trees ...,