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18S and COI sequence data from cross-lab reproducible metabarcoding study

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Research Data Australia2024-08-03 收录
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https://researchdata.edu.au/18s-coi-sequence-metabarcoding-study/1701318
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Samples were collected from 4 harbour sites (Aus, NZ, USA & Canada) and sent to 12 labs for 18S and COI PCR. The dataset is the demultiplexed (by lab) data from this study.\nLineage: The source material was homogenised and give to 12 labs for DNA prep and PCR extraction/amplification using both fused and two-step primers. The resulting DNA was sequenced at Guelph and Curtin. The sequence files were then run together through a CSIRO amplicon pipeline so that differences between labs could be analysed and reproducibility asssesed.
提供机构:
Commonwealth Scientific and Industrial Research Organisation
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