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Effect of in vitro transcription time on the fidelity of T7-based RNA linear amplification

ChEBI2005-11-03 更新2026-07-21 收录

RNA扩增技术

转录保真度优化

数据链接：

https://www.ebi.ac.uk/biostudies/ArrayExpress/studies/E-GEOD-3558 数据链接链接失效反馈

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资源简介：

Effect of in vitro transcription time on the fidelity of T7-based RNA linear amplification

应用场景：

创建时间：

2005-11-03

RNA扩增技术

比较转录组分析

We tested the performance of three methods for amplifying single-cell amounts of RNA under ideal conditions: T7-based in vitro transcription; switching mechanism at 5' end of RNA template (SMART) PCR

NIAID Data Ecosystem80

Additional file 4: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing

单细胞RNA测序

RNA扩增技术

Number of detected genes for low-depth in silico samples. As Fig.Â 2a, except that each dilution replicate has been subsampled to a depth of 500,000 unique genic reads. (XLS 74 kb)

Figshare2016-12-14 更新40

Additional file 1: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing

单细胞RNA测序

RNA扩增技术

Control dataset sample identification, protocol information, and RNA sequencing stats. Experimental group, protocol information and RNA sequencing statistics for each sample used in primary analyses.

NIAID Data Ecosystem30

Effect of the amount of input total RNA on T7 amplification

RNA扩增技术

基因表达分析

Zhao et al. Amplification Table 9 This experiment was designed to evaluate the effect of the amount of input total RNA on the fidelity, reproducibility, and yield of T7 based RNA linear amplification.

NIAID Data Ecosystem40

Additional file 5: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing

单细胞RNA测序

RNA扩增技术

Gene detection logistic regression model. See model details in Methods . Abbreviations: M expected number of input molecules; L gene length (kilobases); G gene GC content; S strength of gene local sec

Figshare2016-12-14 更新30

Effect of in vitro transcription time on the fidelity of T7-based RNA linear amplification

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