Regioselectivity and Stereospecificity of nTPL, rTPL and 6His-rTPL.
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The vicinity index (V.I.) and the stereospecificity index (S.I.) of the three TPL forms were calculated using the following definitions: V.I. = [A1,3−½ (A2,3+A1,2)]/[A1,3+½ (A2,3+A1,2)] and S.I. = (A2,3−A1,2)/(A2,3+A1,2) at two surface pressure values (15 and 25 mN.m−1), where A1,3, A1,2 and A2,3 are lipase activities measured with 1,3-sn-dicaprin; 1,2-sn-dicaprin and 2,3-sn-dicaprin, respectively.
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2015-12-02



