five

Zbtb46 dependent differentiation of embryonic stem cell derived progenitors

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https://www.ncbi.nlm.nih.gov/sra/SRP273023
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We analyzed the effects of Zbtb46 induction in murine embryonic stem cell derived progenitors using a doxycycline-inducible transgenic cell line. Five-day differentiated embryonic stem cells were magnetically separated and the Flk1+ cells were further cultivated in the presence or absence of Zbtb46. To define the early impact of the transgene, cells were harvested after 24 hours; in addition, the transcriptomic profile was also determined after 3 days of differentiation. We employed a genome-scale gene expression analysis with RNA sequencing testing three biological replicates (together 15 samples).
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2021-06-02
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