MicroRNAs Associated with AGL6 and IAA9 function in tomato fruit set

Fruit set is triggered after ovule fertilization, as a consequence of the downregulation of ovary growth repressors, such as the tomato transcription factors Auxin/indole-3-acetic acid 9 (IAA9) and Agamous-like 6 (AGL6). We produced small RNA libraries from IAA9- and AGL6-silenced ovaries to identify miRNAs differentially expressed in IAA9- and AGL6-silenced ovaries as compared with unpollinated control ovaries. The identified miRNAs represent a pool of regulatory sRNAs potentially involved in tomato fruit initiation. Overall design: Gene expression profiling analysis of small RNA-seq data for tomato wild-type ovaries and AGL6- and IAA9-silenced ovaries. Each small RNA library was obtained from total RNA isolated from a pool of ovaries after treatment with exogenous dsRNA.