scRNA- and TCR-Seq of mouse T cells from WT and TIGIT KO mice at steady state and upon LCMV infection

The experimental setup is aimed at understanding the function of the co-inhibitory receptor TIGIT in T cells. We compared sorted T cells (CD8+ T cells, CD4+Foxp3- T cells, CD4+Foxp3+ Tregs) from WT and TIGIT KO mice at steady state (naive mice) and following LCMV infection (day 10, LCMV clone 13). Cell were collected form spleen or lung.