TGF-β specifies TFH versus TH17 cell fates in murine CD4+ T cells through c-Maf (Seq02, bulk-RNAseq). TGF-β specifies TFH versus TH17 cell fates in murine CD4+ T cells through c-Maf (Seq02, bulk-RNAseq)
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA814714
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Naive murine CD4+ T cells from GREAT/SMART-17A mice were cultured under Th1 or Tfh(1 ng/ml TGF-β)-polarizing conditions in 96-well plates coated with anti-CD3/anti-CD28 for 3.5 days; sorted by flow cytometry on IFNg+ (Th1), or CXCR5-IL17A+ (Th17) and CXCR5+IL17A- (Tfh); and subjected to bulk RNA-seq. Overall design: Seq02: bulk RNA-seq: Th1IFNg+, Tfh(1)CXCR5+IL-17A–, Tfh(1)CXCR5+IL-17A–
创建时间:
2022-03-10



