Whole transcriptome sequencing of testis, ovary, and accessory glands of Drosophila species. Drosophila

Differences in gene expression account for the majority of phenotypic differences between males and females of sexually dimorphic species. Thus, accurate identification and characterization of sex-biased gene expression is crucial for understanding the occurrence and evolution of sexual dimorphism. Most sex-biased gene expression occurs in the gonads, sex-specific organs. This study is designed to generate samples for accurate and comprehensive identification and characterization of sex-biased gene expression in several species of Drosophila. We sequenced testis, ovary, and accessory gland samples from D. pseudoobscura and D. ananassae and are in the process of sequencing the remaining species.