Clostridium tetani biomarkers for culture condition

We used RNA-seq to determine Clostridium tetani gene expression changes in response to culture conditions and time. Changes in response to time were more pronounced than those in response to culture conditions. The tetanus toxin gene is always highly expressed but does show expression changes between culture conditions. These results may become part of an approach to reduce animal testing during vaccine manufacturing. Overall design: 39 samples, comprising 6 growth conditions and 5 time points, with some replicates.