Analysis of transcriptomes of germinal center (GC) B cells derived from miR-155-sufficient and -deficient mice at the single-cell level [scRNA-seq]

To investigate the impact caused by miR-155 ablation, GC B cells were sorted by flow cytometry and used to analyze transcriptomic profiles. Splenic B cells derived from miR-155-sufficient or -deficient mice expressing HEL-specific B cell receptor together with endogenous cMyc-GFP reporter cassette and AID-cre-hCD2 were adoptively transferred into congenic mice, followed by immunization with HEL3x mutant conjugated with sheep red blood cells (SRBCs). After seven days, Donor-derived GC-B cells were sorted and used for the scRNA sequencing library.