RNA-seq analysis of Men1-/- versus Men1wt MN1-driven AML

Purpose: To characterize transcriptional changes associated with homozygous inactivation of Men1 in MN1 driven AML Methods: In vivo MN1 transformed cells were transduced with Cre-vector to inactivate Men1 and injected into sublethially irradiated syngeneic recipients. Men1-/- and Men1wt MN1-driven leukemic cells were isolated from moribund recipients and plated in methylcellulose for 7 days before RNA was extracted. Results: Men1-/- MN1-driven leukemic cells failed to propagate leukemia in most secondary recipients, in comparison with Men1wt MN1-driven. In vitro, cells had lost their colony forming activity. Gene expression analysis revealed a downregulation of the MN1 leukemic expression program. Conclusions: Men1 regulates long term maintenance of MN1-driven leukemia. Overall design: Men1-/- and Men1wt MN1-driven leukemic cells were isolated from moribund mice and plated in methylcellulose for 7 days. mRNA from murine Men1-/- and Men1wt MN1-driven AML was submitted for RNAseq