Supplementary Figure 30 and 34 Fluorescence microscopy and bright-field images of the 4-component assembly confirm incorporation of IgG-Alexa Fluor 568 or AtzBSH2 into assembly

Supp Figure 30: <b>Fluorescence microscopy and bright-field images of the 4-component assembly</b> (AtzAM1, AtzCM1, ProteinA-SH2, and antibody, along with extended linker versions of AtzA and AtzC) <b>confirm incorporation of IgG-Alexa Fluor 568 into assemblies.</b> (A) Fractal assembly in DIC and (B) fluorescent image of fractal indicating incorporation of antibody into assembly. (C) Globular assembly in DIC and (D) fluorescent images of globular assembly indicating incorporation of antibody into assembly. The depiction of a fractal and globular topology is easily distinguishable in these images. Supp Figure 34: <b>Fluorescence microscopy and bright-field images of the 3-component assembly confirm incorporation of AtzBSH2 into assembly while bright-field images confirm the fractal-like nature of the 2-component assembly. </b>(A and B) 3 µM AtzAM1, 1 µMAtzBSH2 dye labeled with Alexa FluorTM 647, 2 µM AtzCM1 image shows AtzBSH2 incorporation into 3-component assembly at various locations (C to H) 3 µM AtzAM1 and 2 µM AtzCM1 assembly images depict fractal-like assembly structure.<br>