Thermodynamic and kinetic parameters for the binding of ligands to SaHPPK, as determined by ITC<sup>a</sup> and SPR<sup>a</sup>.
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aValues are the means ± the standard deviation for at least three experiments. See Materials and Methods for sample concentrations used. All ITC and SPR experiments were performed at 298 K and 293 K respectively. bConcentration of AMPCPP was 1 mM in both HPPK (cell) and ligand (syringe) solutions. cNo data. dConcentration of ATP was 5 mM. e8-mercaptoguanine.
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2012-01-19
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