B cell costimulatory molecule expression in the lungs and spleens of naive and cockroach allergen challenged mice at various time points post-challenge.

Mice were euthanized at various time points during chronic allergen sensitization and challenge. Lungs and spleens were removed, enzymatically digested, and the expression of the various markers determined by flow cytometry. The median fluoresence intensity (MedFI) of CD40, etc. by CD19+ cells from naive, ip/sc sensitized (d13), single intranasal challenge (d15), and chronically challenged (d37) mice are shown. Bars represent the mean of 5 mice per group ±SEM. * = p<0.05 vs. naïve. ** = p<0.01 vs. naïve. Similar results were observed in two independent experiments.