Analysis of tumor-infiltrating leukocyte subsets 3 weeks after 4T1 cell inoculation.
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Tumors were excised and digested by collagenase IV for 2 hrs at room temperature. Cell suspensions were filtered and red blood cells were depleted with ACK solution. Cells were stained by monoclonal antibodies against mouse CD45 labeled by PECy5.5, CD11b labeled by PE, Ly6G labeled by FITC or F4/80 labeled by FITC, and the expression of each molecule was analyzed using a FACScan flow cytometer. n = 3, *p<0.05, **p<0.01, ***p<0.001.
创建时间：
2013-03-18
