Summary of PCR validations of rearrangement events.

PCR products for each primer configuration were scored as present (+), absent (–), or ambiguous (*) based on their migration on a 1.5% agarose gel (Fig. S2). Present scores indicate very bright bands of the expected size for both of the blocs spanning each breakpoint of an event. Absent scores indicate the lack of a bright band of the expected size for one or both blocs. The ambiguous score indicates unclear results. Event sizes are the number of base pairs in the WI genome internal to two mate blocs.