Membrane binding controls the ATPase cycle and localization of MinD in <em>Bacillus subtilis</em>

This dataset includes all analysis done for this paper regarding single-molecule localization microscopy (SMLM) and single-particle tracking (SPT). It contains two main folders, \"Figure 6\" and \"Figure 7\", which contain all relevant data for the corresponding figures in the article. The folders and their structures were choosen in a way that it can be directly used to analyse the data in the free software tool \"SMTracker 2,\" which was used to create the data for Figures 6 and 7 as well as Tables 4 and 5. Furthermore, it contains the raw localization tables of all SMLM data as well as all tracking data from SPT. , , # Membrane binding controls the ATPase cycle and localization of MinD in *Bacillus subtilis* Dataset DOI: [10.5061/dryad.95x69p91b](https://doi.org/10.5061/dryad.95x69p91b) ## Description of the data and file structure The dataset was generated to investigate the dynamics and membrane interactions of the cell division protein MinD in *Bacillus subtilis* using single-molecule localization microscopy (SMLM) and single-particle tracking (SPT). Halo-tagged MinD was expressed in wild-type, ΔminC, and ΔminJ genetic backgrounds to assess the contributions of MinC and MinJ to MinD mobility, membrane association, and spatial organization in vivo. Furthermore, ATPase mutants of MinD (G12V, K16A, and D40A) as well as a membrane binding mutant (I260E) were investigated and compared to the wild-type. Time-lapse fluorescence imaging was performed at high temporal resolution to capture individual Halo-MinD trajectories in living cells. The resulting datasets were analyzed to extract diffusion coef..., ,