RNA-seq of gene expression profile after endothelial cell-specific CD147 deletion in mouse cortex

The roles of CD147 in the BBB integrity in the healthy circumstance have not been fully explored. By constructing endothelial cell-specific CD147 knockout (EC-KO) mouse (cre-loxp system), we tried to comprehensively analyze the transcriptomic changes after CD147 deletion on mouse cortex endothelial cells (BECs). Mouse BECs were isolated after enzyme digestion in 4 groups. After 7 days culture, the samples were performed by RNA-seq. The grouping factors contained both genotype (CD147 EC-KO versus WT) and age (6-7 months versus 8-12 weeks). Samples were pooled from 8-10 mice for each group.