Dataset for bacterial community and metagenome in soils affected by straw, biochar and ash
收藏科学数据银行2022-04-02 更新2026-04-23 收录
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资源简介：
Three treatments of soil experiment were set up. The straw and the biochar and ash made from equal amount of straw were amended into soils, respectively. We aimed to investigate the soil bacteria community and metagenome driven by different forms of the straws. （1）After DNA extraction, Primers 505F (5'-GTGYCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACNVGGGTWTCTAAT-3') were used to amplify 16 s rRNA genes V3-V4. Purified product were sent to biological company for sequencing. Data quality control and split were conducted to obtain the clean data (1 fastq file per sample).（2） High quality DNA samples were sent to biological company for the construction of 350 bp gene library, and then paired end 2 × 150bp sequencing was performed on an Illumina HiSeq X Ten platform. Approximately 12 Gb of raw data were obtained for each sample. After quality control, two read files, fq1 and fq2, were given for each sample.
提供机构：
Institute of Urban Environment, Chinese Academy of Sciences
创建时间：
2022-03-18
