Transcriptional profile of ecdysoneless mutants and Wild Type controls

Comparisons of temperature sensitive mutants (ecdysoneless) at permissive and restrictive temperatures to identify genes under control of ecdysone hormone. Also, Wild Type Strain (Canton S), controls for temperature sensitive genes, are included at the temperature used for restrictive temperatures. Modified Round Robin design with dual colors and direct hybridization comparison between each strain and temperature variable. A representative dye swap was conducted for the Wildtype strains to determine whether dye intensity posed a significant/confounding artifact.