Molecular mechanism of EPC1-PHF1 fusion protein (CUT and RUN-seq Homo Sapiens)

In this study, we characterize the fusion protein produced by the EPC1-PHF1 translocation in Low Grade Endometrial Stromal Sarcoma (LG-ESS) and Ossifying FibroMyxoid Tumors (OFMT). We express the fusion protein and necessary controls in K562 Cells. The fusion protein assembles a mega-complex harboring both NuA4/TIP60 and PRC2 subunits and enzymatic activities and leads to mislocalization of chromatin marks in the genome, linked to aberrant gene expression. Overall design: CUT and RUN-sequencing (anti-HA) in K562 cells expressing HA tagged EPC1-PHF1 fusion protein or tag only.