Single cell RNAseq of fibroblastic reticular cells and vascular smooth muscle cells from mesenteric and peripheral murine lymph nodes at different developmental stages

The interaction of immune cells in the lymph node microenvironment depends on the infrastructure and molecular cues provided by fibroblastic reticular cells (FRCs). In addition, concentric layers of still poorly defined mural cells, including vascular smooth muscle cells (VSMCs), are involved in positioning and regulating immune cell interactions. Here, we used high-resolution confocal microscopy, flow cytometry, single cell transcriptomics and trajectory analysis to delineate VSMC and PRC phenotypes and differentiation during development in different LN entities.