Elucidation of High-molecular weight glutenin C-terminal truncation

MS-MS data supporting the identification of C-terminal truncation of Dy and Bx high-molecular weight glutenin subunits (HMWGS) in wheat. HMWGS Dy10 and Bx7* were purified from the Canadian bread wheat variety Carberry. Refer to manuscript for details (see Related Links, below).
Data were acquired on a Waters G2 Synapt in MS-MS mode.
File index:
Direct infusion of peptide standards (Dy10 intact, Dy10 truncated, Bx7* Intact, Bx7* truncated). Acquisitions in MS-MS mode with CE (trap) switched on halfway through run:
File Description
20230417 DY10 IN MS_02.raw m/z = 1282.615, CE = 65
20230417 DY10 IN MS_03.raw m/z = 855.413, CE = 31
20230417 DY10 IN MS_04.raw m/z = 641.811, CE = 17 (Moved CE around in last 10 scans)
20230417 DY10 TR MS_02.raw m/z = 1003.975, CE = 60
20230417 DY10 TR MS_03.raw m/z = 669.652, CE = 25
20230417 INF BX7S IN MS-MS_01.raw m/z = 1332.173, CE = 80
20230417 INF BX7S IN MS-MS_02.raw m/z = 1332.173, CE = 85
20230417 INF BX7S IN MS-MS_03.raw m/z = 888.451, CE = 37
20230417 INF BX7S IN MS-MS_04.raw m/z = 888.451, CE = 30
20230417 INF BX7S TR MS_02.raw m/z = 1003.993, CE = 47
20230417 INF BX7S TR MS_03.raw m/z = 669.664, CE = 25
20230417 LEUENK_01.raw MS1 only, LeuEnk for mass correction

LC-MS-MS of sample digests using CE as optimized above:
File Description
20230420 Dig_Bx7s_HDms-ms_01.raw Bx7* digest (rLysC)
20230420 Dig_Dy10_HDms-ms_01.raw Dy10 digest (rLysC)
20230420 PEP_Bx7s_I_HDms-ms_01.raw Bx7* intact standard peptide
20230420 PEP_Bx7s_T_HDms-ms_01.raw Bx7* truncated standard peptide
20230420 PEP_Dy10_I_HDms-ms_01.raw Dy10 intact standard peptide
20230420 PEP_Dy10_T_HDms-ms_01.raw Dy10 truncated standard peptide

All samples pyridylethylated prior to analysis.