Transcriptome sequencing of middle ear epithelial cells treated with lps

Lipopolysaccharide, as the main virulence factor of the outer membrane of Gram-negative bacteria, can drive the release of pro-inflammatory cytokines by activating the Toll-like receptor 4/NF-kB signaling pathway. It is the core tool for simulating bacterial infectious inflammation in the experimental otitis media model. This study aims to analyze the molecular mechanism of bacterial otitis media by integrating the transcriptomic system.