Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer

Zenodo2023-05-25 更新2026-04-07 收录

冷冻电子叠层衍射成像

单颗粒结构解析

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Advances in cryogenic transmission electron microscopy have revolutionised the determination of many macromolecular structures at atomic or nearatomic resolution. This method is based on conventional defocused phase contrast imaging. However, it has limitations of weaker contrast for small biological molecules embedded in vitreous ice, in comparison with cryo-ptychography, which shows increased contrast. Here we report a single-particle analysis based on the use of ptychographic reconstruction data, demonstrating that three dimensional reconstructions with a wide information transfer bandwidth can be recovered by Fourier domain synthesis. Our work suggests future applications in otherwise challenging single particle analyses, including small macromolecules and heterogeneous or flexible particles. In addition structure determination in situ within cells without the requirement for protein purification and expression may be possible.

提供机构：

JEOL Ltd, Tokyo, Japan.; The Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, UK.; National Laboratory of Solid State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, China.; Department of Materials, University of Oxford, Oxford, UK.; Department of Physics, University of Warwick, Coventry, UK.; Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.

创建时间：

2023-05-25