Marcel Mettlen, Sandra L. Schmid (2010) CIL:10136, Homo sapiens, epithelial cell, human retinal pigmented epithelial cell. CIL. Dataset

Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm

本数据集展现了包裹有网格蛋白的凹斑动态变化。通过总内反射荧光显微镜（TIRF-FM）技术，对稳定表达标记有EGFP（EGFP-LCa）的网格蛋白轻链a（clathrin light chain a）的人视网膜色素上皮（RPE）活细胞进行了观察。实验采用尼康100x 1.45 NA物镜（Nikon），搭载于尼康TE2000U倒置显微镜（Nikon），并连接至14位制式操作的Hamamatsu Orca II-ERG相机。该单通道TIRF-FM电影展示了凹斑在腹侧质膜上的动态变化，采集帧率为1秒，曝光时间为70毫秒，持续10分钟。像素大小为67纳米。