CheW and CheA counts per TD show different trends.

(A) For complexes with rest groups, the number of CheW molecules per TD is independent of complex size. For species with no rest groups, this ratio increases with the number of TDs in the species. Raising the expression level of CheW results in the formation of larger complex sizes. (B) The number of CheA dimers per TD shows two opposing trends with respect to complex size. As the ratio is highest for single TDs with CheA2-including rest groups, raising the expression level of CheA results in smaller complex sizes but an increased number of CheA molecules contributing to the signal amplitude.