Stem cells utilize m6A-mediated decay via YTHDF2 to regulate neural differentiation and promote pluripotency

The m6A methylation pathway has previously been shown to influence pluripotency. YTHDF2 is an RNA binding protein that interacts with m6A-modified RNAs to facilitate their degradation. We sought to identify the cohort of RNAs targeted by YTHDF2 in human induced pluripotent stem cells (iPSC). Overall design: To investigate the impact of YTHDF2 on relative transcript abundances in human iPSCs, we analyzed RNA samples of cells subjected to control or YTHDF2 siRNA-mediated depletion and compared their transcriptional profiles. Three replicates of control and four of YTHDF2 knockdown were evaluated for a total of seven samples.