Gene Regulation by Histone Deacetylase 7 During Invariant Natural Killer T Cell Development

We have found that the signal-dependent chromatin modulator Histone Deacetylase 7 interacts with and corepresses the signature transcription factor of invariant natural Killer T (iNKT) cells, ZBTB16. This study was designed to determine the global effect on transcription of a gain-of-function mutant of HDAC7 during iNKT cell development and function. Va14/Ja18 T cell receptor transgenic thymocytes and splenocytes, which develop preferentially into iNKT cells, were profiled by RNA-seq with and without expression of a gain-of-functon HDAC7 transgene (HDAC7dP) There are 18 samples overall, consisting of 3 replicates each of CD4 splenoctes and CD4SP thymocytes, with either no transgene or with the V-alpha14/j-alpha18 TCR transgene +/- the HDAC7 deltaP transgene. The Va14/Ja18 TCR transgenic cells were sorted using CD1D/aGalCer tetramers to identify NKT cells.