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DNA sequence and SNP data for species of 'Conyza' (Erigeron, Asteraceae) introduced to Australia

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Research Data Australia2025-12-20 收录
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https://researchdata.edu.au/dna-sequence-snp-introduced-australia/3752191
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资源简介:
Target capture sequencing was conducted on 48 specimens of five putative species of 'Conyza' presumed to be introduced to Australia: Erigeron bilbaoanus (= floribundus), Erigeron canadensis, Erigeron pusillus (= canadensis var pusillus, or Conyza parva), Erigeron bonariensis, and Erigeron sumatrensis, to clarify species boundaries and which entities occur in Australia. The data collection contains the concatenated supermatrix of sequence data used for likelihood phylogenetics, gene trees used for two-step coalescent analysis, the phylogenetic trees in Newick format, VCF files of SNP variants, and the geno file used for sNMF analysis. Raw reads will be published in a sequence read archive.\nLineage: DNA extraction from herbarium specimens, enzymatic fragmentation, library prep, target capture using Angiosperms 353 bait kit, DNA sequencing using NovaSeq X with 2 x 150 paired-end chemistry. DNA sequence data: assembly with HybPiper, orthology inference with Paragone, gene tree inference and phylogenetic analysis of concatenated supermatrix using IQ-TREE, two-step coalescent phylogenetic analysis using ASTRAL-IV. Single Nucleotide Polymorphism data: variant calling using freebayes, filtering with VCFtools.
提供机构:
Commonwealth Scientific and Industrial Research Organisation
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