Movie analysis reference table and Statistical analysis size and motion results.

Spreadsheet 1: This table shows the names of all LLSM datasets analyzed in this work, as well as a note for each dataset that is included as a supplementary movie. Also noted in the table is the temporal resolution of the datasets, whether they were included in motion analysis (Δt~6.12 sec or less), and the number of ectoderm and skeletogenic mesoderm vesicles used in the size analysis (first-frame) and the motion analysis (total). Spreadsheets 2–7: These spreadsheets contain the statistical comparisons for each size and motion features measured from the vesicle tracks. Comparisons are run across experimental conditions (Control/VEGFR Inhibition) and embryo regions (Ectoderm/Skeletogenic Mesoderm). Each sheet in the dataset contains the comparison of a single measured feature (e.g., size, velocity, etc.) across conditions and regions. In the case of spicule-relative analysis, comparisons are run across groups of vesicles at different average distance from the spicule binned in 1μm groups. Spreadsheets 8–9: These sheets contain comparisons of cytoskeletal remodeling experiments under both experimental conditions (Control/VEGFR Inhibition) and in both embryo regions (Ectoderm/Skeletogenic Cells). For sheets 2–9, the Kruskal-Wallis test is used to establish differences in centrality between groups, followed by a post-hoc Dunn-Sidak test to establish pairwise differences. A brief description of the comparison is included at the bottom of each sheet. Spreadsheet 10: This sheet contains a comparison of average vesicle speed and vesicle diffusion coefficient to vesicle size, the comparison shows minimal correlation between size and velocity or diffusion suggesting that vesicle motion is independent of its size (possibly an active diffusion mode). Spreadsheet 11: Shows a detailed table of the microscope parameters used during imaging per dataset. (XLSX)

工作分析中涉及的所有 LLSM 数据集的名称均展示于第一张电子表格中，且每个数据集均附有备注，备注内容包含作为补充资料的辅助视频。此外，表格中还注明了数据集的时间分辨率、是否包含运动分析（Δt~6.12秒或以下），以及用于大小分析（首帧）和运动分析（总计）的表皮外胚层和骨骼发生中胚层泡的数量。第二至第七张电子表格包含从泡轨迹中测量的每个大小和运动特征的统计比较。这些比较在实验条件（对照组/VEGFR 抑制）和胚胎区域（表皮/骨骼发生中胚层）之间进行。数据集中每张表格都包含单个测量特征（例如，大小、速度等）在条件与区域间的比较。在棘层相对分析的情况下，比较是在棘层平均距离不同且按1μm分组的不同泡组之间进行的。第八至第九张表格包含在实验条件（对照组/VEGFR 抑制）和胚胎区域（表皮/骨骼发生细胞）下进行的细胞骨架重塑实验的比较。对于第二至第九张表格，使用 Kruskal-Wallis 检验来建立组间中心性的差异，随后通过 Dunn-Sidak 随机对照试验来建立成对差异。每张表格底部均包含比较的简要描述。第十张表格包含对平均泡速度和泡扩散系数与泡大小之间进行比较，比较结果表明大小与速度或扩散之间的相关性极小，表明泡的运动与其大小无关（可能是一种活跃的扩散模式）。第十一张表格详细列出了每个数据集成像过程中使用的显微镜参数。（XLSX）