Modulation of multiple gene clusters expression by a single PAS-LuxR transcriptional regulator

PAS-LuxR transcriptional regulators are highly conserved enzymes governing polyene macrolide antifungal biosynthesis. One of such regulators PteF, is located in the polyene macrolide filipin gene cluster from Streptomyces avermitilis and its mutation leads to a drastic decrease in filipin production as well as a severe loss of oligomycin production, an ATP-synthase inhibitor of macrolide structure, and a delay in sporulation. This transcriptomic study aims to understand the regulatory role of PteF and unveil the reach of PAS-LuxR transcriptional regulators in the global metabolism of Streptomyces. Overall design: Two-colour microarray with common reference. The transcriptomes of S. avermitilis NRRL 8165 (WT) and S. avermitilis MUF (DpteF) were compared. Two time points were included: exponential (t1) and stationary (t2) phase. Biological triplicates were performed for each strain/time point. Genomic DNA of S. avermitilis NRRL 8165 was used as common reference.