Insights into the lipid inverse liquid crystalline mesophase behaviour of ionisable lipid nanoparticles encapsulating mRNAs by time-resolved

ESRF Portal2028-01-01 更新2026-04-23 收录

脂质纳米颗粒组装

基因递送系统优化

数据链接：

https://doi.esrf.fr/10.15151/ESRF-ES-2227638373 数据链接链接失效反馈

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资源简介：

Gene therapeutics using mRNAs, DNAs, and siRNAs hold great potential to revolutionise many disease prevention and treatment. The quest for safer, more effective, cell-specific, and organ-targeting delivery system for the nucleic acids still needs significant research effort. This proposal aims to use the ESRF ID02 beamline, in particular its time-resolved, rapid-mixing stopped-flow apparatus, to investigate the lipid nanoparticles (LNPs) as gene delivery systems. In particular, various novel LNP formulations and the benchmark clinical LNP formulation replicates (used in the COVID-19 mRNA vaccines) will be systematically studied at the ID02 beamline in terms of their self-assembly process and structure formation kinetics upon mixing with mRNAs in aqueous solutions or mixing with proteins in serum. The improved understanding of the structure-function relationship will have a significant impact in designing safer and more effective LNPs with cell and organ targeting ability.

提供机构：

CNRS UMR8612, Institute Galien Paris-Saclay, BPC, Bât. Henri MOISSAN, 19 Av. des Sciences, Université Paris-saclay, 91400 Orsay, France; RMIT University, Molecular assembly group/ School of Science, 124 La Trobe St, VIC 3000, 3001 Melbourne, Australia; Olivia Newton-John Cancer Research Institute, Nanomedicine and Gene Therapeutics Laboratory, Level 5, ONJ Centre 145 Studley Rd Heidelbe, 3084, Melbourne, AUSTRALIA; ELI Beamlines, Za Radnicí 835, 252 41 DOLNI BREZANY, Czech Republic; RMIT University, Molecular assembly group/ School of Science, 124 La Trobe St, VIC 3000, 3001, Melbourne, AUSTRALIA

创建时间：

2028-01-01