H3K23ac levels at piRNA clusters and transposons.

Unique mappers from the H3K23ac and H3 ChIP-seq analyses of two replicates of the control (GFP_KD) or the enok knockdown (enok_KD) ovaries mapping to each piRNA cluster or transposon were analyzed using DESeq2. The H3 ChIP-seq data were used as the normalization control for the H3K23ac ChIP-seq data. The results of differential analysis of H3K23ac levels in enok_KD compared with GFP_KD are shown. Transposons or piRNA clusters with decreased H3K23ac levels upon enok knockdown were defined as log2FoldChange < -1 and adjusted p-value < 0.05. Genotypes are as follows: tj-GAL4/+; P{w[+mC] = GAL4::VP16-nos.UTR}CG6325MVD1 / P{VALIUM20-EGFP.shRNA.1}attP2 (GFP_KD); tj-GAL4/+; P{w[+mC] = GAL4::VP16-nos.UTR}CG6325MVD1/P{TRiP.HMS02634}attP2 (enok_KD). (XLSX)