RNA seq analysis of Nfe2 expressing 4T1 and TS/A cells. RNA seq analysis of Nfe2 expressing 4T1 and TS/A cells

We founed enhanced Nfe2 mRNA expression in bone tropic mouse breast cancer cell lines, 4T1.3, grown in a bone cavity, compared with in vitro culture condition. Thus, we examined about the function of Nfe2 in tumor growth in bone microenvironment in this study. Overall design: Total RNA was extracted from mouse breast cancer cell lines 4T1 and TS/A cells which wre transduced Nfe2 gene. The resultant total RNAs were subjected to RNAseq.