BPMV-VIGS targeting iron related genes in Clark (Glycine max)

We used bean pod mottle virus (BPMV) vectors to perform virus induced gene silencing (VIGS) on the soybean (Glycine max) cultivar Clark. Six BPMV-VIGS vectors we created targeting three genes individually, two combinations of two genes in the same vector, and an empty vector to serve as a control. The three individual genes were GmGLU1 (Glyma.03G128300), GmARR4 (Glyma.03G130000), and GmbHLH38 (Glyma.03G130400 and Glyma.03G130600). We used the Gibson Assembly method to create two vectors that combined GmARR4+GmGLU1 or GmbHLH+GmGLU1 in order to target two genes simultaneously. All plants were grown in iron sufficient hydroponics.