Quantification of the phase shifts in control siblings versus <i>lak</i>-/- (<i>lak</i>) larvae kept in DD or submitted to pulses of white light at CT16 or CT21.

The Δphase is the difference between the phase of the two last cycles and the phase of the two first cycles. A phase shift is observed in DD owing to the period that is close to 25 hours which generates a ~1 hour-shift every cycle. Upon a pulse of light at CT16 or CT21 a statistical difference is observed between DD and pulsed sib larvae as well as lak and sib larvae when the pulse of light is applied at CT21 (****, p<0,0001; *, p<0,05 using a Mann-Whitney two-tailed test). For each type of paradigm, (DD, PD and PA) three independent experiments were pooled.