alanine + tRNA(Ala) + ATP => Ala-tRNA(Ala) + AMP + pyrophosphate

AARS2 (mitochondrial alanyl tRNA synthetase) catalyzes the reaction of alanine, mitochondrial tRNA(Ala), and ATP to form Ala-tRNA(Ala), AMP, and pyrophosphate. The AARS2 gene has been identified by computational analysis of the human genome sequence; its function has been inferred from those of the biochemically characterized mitochondrial aspartyl and tyrosyl tRNA synthetases (Bonnefond et al. 2005).

AARS2（线粒体丙氨酸tRNA合成酶）催化丙氨酸、线粒体丙氨酸tRNA（Ala）和三磷酸腺苷（ATP）的反应，形成丙氨酸-tRNA（Ala）、腺苷酸（AMP）和焦磷酸。AARS2基因通过计算分析人类基因组序列而被识别；其功能基于对已生化特征化的线粒体天冬氨酸和酪氨酸tRNA合成酶（Bonnefond等，2005年）的研究而推断。