Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Melanoma Cell MUM2B overexpressing Empty Vector Or LACTB

Methods: mRNA profiles of MUM2B cells stably overexpressing empty vector or LACTB were generated by deep sequencing, in triplicate, using Illumina. featureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene. Differential expression analysis of two groups was performed using the DESeq2 R package(1.16.1). qRT–PCR validation was performed using TaqMan and SYBR Green assays Overall design: mRNA profiles of MUM2B cells stably overexpressing empty vector or LACTB were generated by deep sequencing using Illumina.